Determination of SNP 5mC/T in position Chr1: 245618129 in DNA samples from the human blood by GlaI- and FatI-PCR assay

A.G. Akishev¹, N.A.Netesova², M.A. Abdurashitov¹, S.Kh. Degtyarev¹

¹ SibEnzyme, Novosibirsk
² Epigenlab, Novosibirsk

* corresponding author: Alexander Akishev, SibEnzyme, 2/12 Ak.Timakov Str., Novosibirsk 630117, Russia; Tel: +7 383 3334991; Fax: +7 383 3336853; E-mail: aki@sibenzyme.ru

SNP 5mC/T (Chr1: 245618129) in DNA samples from the human blood of 92donors was studied by GlaI and FatI-PCR assay. The work includes a) isolation of DNA from the blood cells, b) GlaI and FatI-PCR assay of DNA fragment Chr1: 245617889 – 245618464, c) determination of 5mC (Chr1: 245618129) and thymine in DNA preparations and d) comparative analysis of the obtained results.

It was shown that in position (Chr1: 245618129) 43 donors (46,74%) have a heterozygote (5mC/T), 28 donors (30,43%) have homozygote (T/T) and 21 donors (22,83%) have homozygote (5mC/5mC). Thus, taking into account a diploid set of chromosomes in the blood cells, SNP 5mC/T is observed in 99 positions from 184 (53,8%).

The results obtained have shown that cytosine in position Chr1: 245618129 in the most of the blood DNA is methylated.

Keywords: SNP 5mC/T analysis, DNA from human blood, GlaI-PCR assay, FatI-PCR assay

DOI: 10.26213/SE.2019.69.42836

Citation::

Akishev A.G., Netesova N.A., Abdurashitov M.A., Degtyarev S.Kh. Determination of SNP 5mC/T in position Chr1: 245618129 in DNA samples from the human blood by GlaI- and FatI-PCR assay. Epigenet DNA diagnx, vol.1, 2021, p.13-23, DOI: 10.26213/SE.2019.69.42836

This work is available at Creative Commons licence «Attribution-NonCommercial»  4.0 International.

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